LMG - Laboratory of Molecular Genetics
Jan Trka
@email, tel.: (+420) 224 436 580
- Graduated of 2nd Faculty of Medicine and then got his PhD in Molecular Genetics; has worked in Austria and as a postdoc in the UK (Bristol)
- position: head of CLIP, specialises in molecular genetics
- has an interest in the genesis and development of the leukaemic clone, genetic-based stratification of childhood acute leukaemias and minimal residual disease
- would probably still remember the 1990’s lab methods
- motivation: is with CLIP because, well, he co-founded it!
Jan Zuna
@email, tel.: (+420) 224 436 580
- majored in Genetics at the 2nd Faculty of Medicine, where he also stayed for his doctoral studies. Went on to be a postdoc in London,UK.
- position: principal investigator
- has an interest in the origin, development and death of the leukaemic clone, in secondary malignancies, residual disease, genes such as TEL/AML1, BCR/ABL, TEL/ABL and MLL/anything
- works at a computer (nearly all the time) and loves it (sometimes, that is) and thinks he might still be able to find his way to the lab
- motivation: they turned him away at FC Slavia Prague
Júlia Starková
@email, tel.: (+420) 224 436 485
- graduate of Faculty of Science in Košice; got her PhD in Genetics at CLIP
- worked as postdoc in the US.
- position: principal investigator
- interested in L-asparaginase, role of HOX genes in leukaemias, epigenetics
- uses cell cultures, cloning, qPCR, western blot, lucipherase assay
- motivation: lives across the street
Eva Froňková
@email, tel.: (+420) 224 436 489
- graduate of the 1st Faculty of Medicine of Charles University and postgraduate studies in Cell Biology and Pathology at the 2nd Faculty of Medicine of Charles University
- project leader / principal investigator
- she is interested in recombination of immunoglobulin (Ig) and T-cell receptor (TCR) genes in lymphocytes, its role and possible application in lymphoid malignancies, immunodeficiencies and immune dysregulation
- dedicated to developing new methods for monitoring minimal residual disease using next-generation sequencing (NGS) and introducing new approaches to the diagnosis of rare diseases of the hematopoietic and immune systems
- motivation: dr. Stuchlý brews the best tea in the country
Ludmila Boublíková
@email, tel.: (+420) 224 436 485
- graduate of First Faculty of Medicine, Charles Universtiy; got her PhD in Molecular Medicine at Trinity College, Dublin
- position: principal investigator
- not interested in anything anymore, but occasionally tries to escape from oncologic patients to oncogenic molecular aberrations
- works with molecular-biologic techniques and, occasionally, other different methods, as well as with a whole lot of people, whom she kindly and insistently asks to work for her - and for the benefit of humankind
- motivation: sheer curiosity as to what it’s all about
Markéta Kubričanová Žaliová
@email, tel.: (+420) 224 436 580
- graduate of Second Faculty of Medicine, Charles University; got her PhD in molecular genetics at CLIP; worked as a postdoc in Kiel (ALL BFM study centre, Germany)
- principal investigator
- interested in genetic aberrations of childhood ALL, their biological role and their potential use in classification, risk assessment and targeted therapy of ALL
- is responsible for routine molecular-genetic diagnostics of childhood AML and advanced molecular-genetic diagnostics of childhood ALL (SNParray, WES, RNAseq, targeted PCR)
Karolina Škvárová Kramarzová
@email, tel.: (+420) 224 436 485
- graduate of Faculty of Science (M.Sc.) and Second Faculty of Medicine, Charles University (PhD); worked at University of Minnesota Stem Cell Institute
- position: junior principal investigator
- interested in gene therapy and gene editing (CRISPR-Cas9 etc.)
- uses gene editing, qPCR, cloning and mutation analysis
- motivation: doesn’t have to get up early for this work
Lucie Winkowská
@email, tel.: (+420) 224 436 580
- graduate of Czech University of Life Sciences in Prague and postgradual studies at the same university
- position: researcher at CLIP
- interested in almost everything related to NGS
- prepares libraries for NGS and single cell NGS and also takes care of NextSeq
- motivation: wants to find out what all the abbreviations that everyone uses mean
Maryna Vasylkivska
@email, tel.: (+420) 224 436 584
- graduate of National university of food technologies (Kyiv, Ukraine); did her PhD at University of Chemistry and Technology, Prague
- position: researcher at CLIP
- interested in cell cancer metabolism, role of glutamate and other amino acids in L-asparaginase treatment
- works with cell lines, measuring of cellular stress response, metabolic assays and stable isotope tracing
- motivation: occasionally needs to take a break from playing videogames
Kateřina Hložková
@email; tel.: (+420) 224 436 485
- graduate of University of Chemistry and Technology (General and Applied Biochemistry) where she also stayed for her PhD ; internship at Institute of Metabolism and Systems Research, University of Birmingham
- position: postdoc in CLIP
- interested in L-asparaginase, cancer metabolism and trying new methods
- prefers stable isotope tracing but also have to use cloning, lentiviruses, western blots, Seahorse, qPCR and other methods
- motivation: to uncover the secret of post-PCR
Lucie Peterková
@email, tel.: (+420) 224 436 485
- graduate of University of Chemistry and Technology (General and Applied Biochemistry)
- postdoc in CLIP
- interested in cells and gene editing
- works with cells, nucleofection and CRISPR/Cas9
- she is in CLIP because it is close to the pool
Natividad Alquézar Artieda
@email, tel: (+420) 224 436 584
- graduate of University of Barcelona
- worked at Agustí Pi i Sunyer biomedical research institute, Vall D'Hebron biomedical research institute and in Glaxosmithkline, Barcelona
- position: PhD student
- interested in L-Asparaginase and cancer metabolism
- motivation: to make her scientific dream come true
Markéta Racková
@email, tel.: (+420) 224 436 584
- graduate of Second Faculty of Medicine, Charles University (clinical intership in Pediatric Department in Carlsbad Hospital)
- position: PhD student
- interested in gene therapy and methods of gene editing, especially CRISPR-Cas9
- motivation: finally has found her tallness group here, no more standing out
Justina Skořepová
@email, tel.: (+420) 224 436 584
- graduate of Second Faculty of Medicine, Charles University
- position: PhD student
- interested in BCR/ABL positive leukaemias
- works with qPCR and (sc)NGS
- She joined CLIP as she stands behind her motto "It is better never to discover something by research than never to cure someone as an MD"
Kryštof Šeferna
@email, tel.: (+420) 224 436 583
- graduate of Second Faculty of Medicine, Charles University
- position: PhD student
- MD at Department of Paediatric Haematology and Oncology
- interested in rearrangement of antigen receptors, NGS, single-cell sequence & and how it all works
- he is in the lab because got an offer that he does not refuse
Karolína Janoušková
@email, tel.: (+420) 224 436 584
- graduate of Biotechnology of Pharmaceuticals Programme at University of Chemistry and Technology
- position: PhD student
- interested in cancer metabolism and methods of gene editing
- motivation: she wants to know what miracles the cells can do during the experiment
Laura Matoušková
@email, tel.: (+420) 224 436 472
- graduate of Faculty of Science, Charles Univesity, Department of Anthropology and Human Genetics
- position: PhD student
- interested in testicular tumors and gene editing
- motivation: loves riding the Metro from one final station to the other
Kristýna Šoffrová
@email, tel.: (+420) 224 436 472
- graduate of Second Faculty of Medicine, Charles University
- position: PhD student
- interested in neuroblastoma and methods of gene editing
- motivation: was drawn to CLIP by accident and is happy about it now
Matúš Kolárik
@email, tel.: (+420) 224 436 472
- graduate of Faculty of Science, Charles Univesity, Department of Biochemistry
- position: PhD student
- interested in cancer metabolism
- motivation: curious whether he can move a laboratory into a modular cabin
Leona Rezková Řezníčková
@email, tel.: (+420) 224 436 521
- graduate of Faculty of Sciences, Charles University
- position: head lab technician
- interested in minimal residual disease in leukamias, next generation sequencing (NGS), accreditation duties
- works with a computer, with documents of all kinds, sometimes even grabs a pipette
- motivation: she almost is a part of the inventory and so far, she hasn´t been written off...
Taťána Valová
@email, tel.: (+420) 224 436 521
- graduate of University of Veterinary and Pharmaceutical Sciences Brno
- position: lab technician
- interested in monitoring minimal residual disease
- works with qPCR, nucleic acid isolation
- motivation: awesome view from the lab window
Petra Komersová
@email, tel.: (+420) 224 436 521
- graduate of Molecular Biology programme at Faculty of Science, Charles University
- position: lab technician
- interested in monitoring minimal residual disease in leukemias with Ig/TCR rearrangements or any MLL fusions
- motivation: wants to find out if CLIP is really that cool as rumor has it
Nikola Mašová
@email, tel.: (+420) 224 436 521
- graduate of Experimental biology programme at Faculty of Science, Palacký University Olomouc
- position: lab technician
- interested in monitoring minimal residual disease and next-generation sequencing
- prepares samples for AML diagnostics
- motivation: loves pipetting
Petra Rácová
@email, tel.: (+420) 224 436 521
- graduate of Faculty of Chemical Technology, University of Chemistry and Technology Prague
- position: lab technician
- responsible for processing the patient samples for minimal residual disease monitoring
Kristýna Brabcová
@email, tel.: (+420) 224 436 521
- graduate of polytechnology in Prague
- position: lab technician
- interested in monitoring minimal residual disease
- works with qPCR, nucleic acid isolation
- motivation: loves having her picture on the CLIP website
Kristýna Zavadilová
@email,, tel.: (+420) 224 436 521
- graduate of Faculty of Science, Charles University
- position: lab technician
- interested in monitoring minimal residual disease in leukemias
- examines relevant fusion genes, works with qPCR, performs isolation of nucleic acids
- motivation: wants to learn medical terminology
Karolína Šilhová
@email,, tel.: (+420) 224 436 521
- graduate of Laboratory Diagnostics in Healthcare at Faculty of Biomedical Engineering, Czech Technical University Prague
- position: lab technician
- interested in monitoring minimal residual disease
- works with qPCR, performs isolation of nucleic acids
- motivation: is only satisfied with the very best
Daniela Baumgartnerová
tel.: (+420) 224 436 521
- graduate of Molecular Biology and Genetics at Faculty of Science, Masaryk University Brno
- position: lab technician
- interested in monitoring minimal residual disease
- works with qPCR, performs isolation of nucleic acids
- motivation: gets to meet a lot of smart and funny people
CLIP - Laboratory of Flow Cytometry
Ondřej Hrušák
@email, tel.: (+420) 224 436 477
- graduate of 2nd Faculty of Medicine, CU and doctoral programme in immunology; has worked in the US
- position: head of cytometry lab
- holds board certification in clinical immunology and allergology
- interested in genesis, development and immunological diagnostics of leukaemia
- though interested in other areas of immunology, his job as Faculty Dean takes precedence
- motivation: is a cofounder; also, CLIP is within the biking/skiing distance
Tomáš Kalina
@email, tel.: (+420) 224 436 487
- graduate of 2nd Faculty of Medicine, CU; holds PhD in immunology; has worked at FHCRC, Seattle, USA
- position: principal investigator
- interested in all aspects of flow cytometry, affinity proteomics (the cytometric side of), development and deficits of B-cells, leukaemia phenotyping and leukaemogenesis, immunity reconstitution, advanced analyses of cytometric data; works with flow cytometry
- motivation: he’s grown fond of it in each and every way and finds working here quite pleasant
Ester Mejstříková
@email, tel.: (+420) 224 436 477
- graduate of the 1st Faculty of Medicine, CU; holds PhD in immunology; her PhD thesis’ topic was Immunological diagnostics and monitoring of treatment response in childhood haematological malignancies
- position: principal investigator
- interested in diagnostics of childhood haematological and immunological disorders and development of blood elements in bone marrow in relationship to plasticity and maturity of leukaemic cells
- works with computers and their peripherals, i.e. flow cytometers
- motivation: can do 12 hour shifts every day
Karel Fišer
@email, tel.: (+420) 224 436 585
- graduate of Faculty of Science; got his PhD there for ‘Changes in gene expression in progression of Chronic myeloid leukemia’; has worked as postdoc in Newcastle, UK
- position: principal investigator
- interested in analysis of large amounts of data –flow cytometry, proteomic data from bead arrays and bioinformatics in general.
- owns neither white coat, nor a bench, nor a pipette
- motivation: no need to own a white coat or pipette
Naděžda Brdičková
@email, tel: (+420) 224 436 583
- PhD in immunology (Faculty of Science, Charles University and Institute of Molecular genetics, Academy of Sciences)
- worked at UCSF, USA as a postdoc
- takes care of the EuroFlow Quality Assessment project
- motivation: great people, meaningful work
Jan Stuchlý
@email, tel: (+420) 224 436 489
- Graduate of Faculty of Mathematics And Physics, CU
- position: PhD student
- Has worked in France on optimising anti-tumour therapies
- Interested in immunity reconstitution, game theory, mathematical programming and such
- motivation: enjoys his work
Barbora Vakrmanová
@email, tel.: (+420) 224 436 477
- graduate of 2nd Faculty of Medicine, CU
- position: PhD student
- interested in leukemias with switch into monocytic lineage, sometimes without the switch as well
- works with PC and flow cytometry, occasionally these machines don´t work with her
- motivation: once she had come around by accident and liked it here so much that she hasn´t left since
Tereza Podolská
@email, tel.: (+420) 224 436 585
- graduate of the Faculty of Science, Charles University, Immunology
- position: PhD student
- interested in bioinformatics analysis of big cytometric data & automatic immunophenotypisation
- motivation: CLIP is just the best. Also, it is well accessible by metro
Jitka Stančíková
@email, tel.: (+420) 224 436 583
- graduate of Faculty of Science, Charles University, where she later got her PhD
- works with ambiguous leukemias and their flow cytometry procesing
- motivation: being here is part of my therapy to overcome my lifelong fear of cytometers
Daniela Kužílková
@email, tel.: (+420) 224 436 477
- graduate of Faculty of Science, Charles University
- interested in mass cytometry in tumors
- works mainly with PC and flow and mass cytometry
- motivation: had been turned away at McDonald’s for the lack of qualification
Violeta Bakardjieva-Mihaylova
@email, tel.: (+420) 224 436 472
- graduate of the Faculty of Science, Charles University
- motivation: there is always enough chocolate here
Daniel Thűrner
@email, tel.: (+420) 224 436 413
- graduate of Secondary Medical School, Kolín
- position: head lab technician
- his work is his hobby
- keeps the lab going smoothly; helps newcomers get oriented
- motivation: the well-being of CLIP
Pavla Luknárová
@email, tel.: (+420) 224 436 413
- graduate of Masaryk Secondary School of Chemistry, Prague
- studies at Faculty of Environmental Sciences, Czech University Of Life Sciences, Prague
- position: lab technician
- motivation: CLIP is fun!
Pavel Semerák
@email, tel.: (+420) 224 436 413
- graduate of Secondary Technical School of Food Technology, Prague
- position: lab technician
- interested in what’s hot in flow cytometry
- does mainly sorting of cell subpopulations
- motivation: knows no other trade; plus, the canteen rules!
Sára Hejnicová
@email, tel.: (+420) 224 436 487
- graduate of Faculty of Arts, Charles University
- assistant to grant projects in CLIP
- likes nice people around, if any
- motivation: even though she doesn't understand it at all, it's fascinating and makes sense
Assistant
Lucie Galdová
@email,tel.: (+420) 224 436 580
- graduate of the Vocational College of Economics, major in Financial Management and Accountings
- asistant to the head of the laboratory centre
- interested in all that comes around
- works with all that comes around
- motivation: proudly represents the Valachian population and spreads moravian language
Maternity/Parental leave
Veronika Kanderová
- graduate of Faculty of Science, CU; holds PhD in immunology from CLIP
- position: postdoc
- holds certification in allergy and clinical immunology; participates in the diagnosis of immune disorders
- interested in proteomics and phosphoproteomics in leukaemias and primary immunodeficiencies
- works with polychromatic flow cytometry and with immunologic tests, likes cultivation and stimulation of cell cultures
- motivation: apparently, the cytometer’s laser beams will keep her young forever
Veronika Grecová
- graduate of the Secondary Medical School, Prague
- position: lab technician
- interested in minimal residual disease in leukamias, MLL gene rearrangements
- works with qPCR, nucleid acid isolation
- motivation: used to think MLL was short for Major League Lacrosse
Eliška Potůčková
- graduate of the Pharmaceutical faculty, Charles University where she also got her PhD in Patobiochemistry and Xenobiochemistry
- position: postdoc
- interested in the ERG gene in ALL
- works with cell cultures, cloning, western blott, luciferase assay
- motivation: CLIP is located close to her home
Andrea Rennerová
- graduate of the Faculty of Biomedical Engineering and graduate of the University of Chemistry and Technology
- position: lab technician
- interested in monitoring minimal residual disease, next generation sequencing with IonTorrent and Illumina
- motivation: a lot of fun in CLIP
Lenka Hovorková
- graduated from the Faculty of Science, Charles University
- position: PhD student, IT geek
- interested in BCR/ABL positive leukaemias
- works with LT-PCR, electrophoresis, R, Linux and now also with her baby boy
- motivation: wants to know what’s behind all without-handle door
Marina Bakardjieva
- graduate of the Faculty of Science, Charles University, Immunology
- position: PhD student
- interested in immunofenotyping and functional testing of lymfocytes in primary immunological deficiencies
- motivation: CLIP allows her to jumping into the unknown
Kateřina Hrušková
- graduate of the Faculty of Food and Biochemical Technology UCT Prague
- position: lab technician
- interested in monitoring minimal residual disease
- works with qPCR, nucleic acid isolation
- motivation: gets enough sleep on her way from work
Martina Zwyrtková
- graduate of the Faculty of Science, University of South Bohemia
- position: researcher
- interested in HOX genes in leukaemias
- works with cell lines, qPCR and western blot
- motivation: when she starts singing people join in
Kristýna Králíková
- graduate of Faculty of Science, Charles University
- position: lab technician
- interested in monitoring minimal residual disease
- works with qPCR, nucleic acid isolation
- motivation: wanted to find out whether coffee is as necessary as other people claim
Marta Rozumová
- graduate of Czech University of Life Sciences in Prague
- position: lab technician
- motivation: because I scare here
Danica Pechová
@email, tel.: (+420) 224 436 521
- graduate of Bioanalytical laboratory diagnostics in health care at Charles University in Hradec Králové
- position: lab technician
- interested in monitoring minimal residual disease in leukemias
- examines relevant fusion genes, works with qPCR, performs isolation of nucleic acids
- motivation: learning new skills at CLIP
Michaela Nováková
@email, tel.: (+420) 224 436 477
- graduate of 2nd Faculty Of Medicine, CU
- position: postdoc
- studies mechanisms of bone marrow failure, immunophenotyping of leukemia, minimal residual disease and technical aspects of cytometry
- works with flow cytometry including spectral cytometry
- motivation: wants to create a record in clicking using pc mouse
Alumni
CLIP PhD graduates:
Michael Svatoň, MD, Ph.D.
Kateřina Rejlová, Ph.D.
Julius Lukeš, MD, Ph.D.
Lucie Slámová, MD, Ph.D.
Violeta Bakardjieva-Mihaylova, Ph.D.
Olga Zimmermannová, MD, Ph.D.
Ondřej Pelák, MD, Ph.D.
Ivana Heřmanová, Ph.D.
Michaela Biehl (Kotrová), MD,Ph.D.
Jana Volejníková, MD, Ph.D.
Michal Zápotocký, MD, Ph.D.
Karel Švojgr, MD, Ph.D.
Jozef Madžo, Ph.D.
Ondřej Krejčí, MD, Ph.D.
Lucie Šrámková, MD, Ph.D.
CLIP MD graduates:
Kateřina Mužíková
Markéta Šimčíková
Alena Jelínková
Alena Musilová
Vendula Šinkorová
Felicita Garcia N'Dua
David Novák