Our technology

Our technology

Devices for molecular genetics

           

NextSeq

  • Manufacturer: Ilumina
  • In CLIP since 2015
  • The NextSeq Series desktop sequencing system provides exome, transcriptome and targeted sequencing
  • Uses fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands
  • Produces up to 400M reads in single workflow with read length 75-150bp

 


 

Ion Personal Genome Machine® (PGM) Sequencer 

 

  • Manufacturer: Life Technologies
  • In CLIP since 2012
  • This new generation benchtop sequencer works on the massively parallel sequencing principle. During sequencing, this system detects hydrogen ions that are released after nucleotide is incorporated into the DNA string. Information obtained this way is then converted into digital form.
  • Works with 3 types of semiconductor chips. Using the biggest chip, up to 1 Gb of sequencing data can be generated within several hours.
  • Produces reads 35 - 400 bp long with a 99 % accuracy
  • We would like to use this system for a detection of minimal residual disease based on Ig/TCR rearrangements 

 

      

 

   

 

 

 

Agilent 2100 Bioanalyzer

    • In CLIP since 2005 and another unit since 2009
    • We use this system to locate new targets for a detection of minimal residual disease in patients with acute lymphoblastic leukemia





LightCycler480 II

    • Manufacturer: Roche
    • Has been in CLIP since several years
    • We use LightCycler480 II for searching for point mutations that increase the probability of thrombophilic disease




TaqMan 7500 Fast Real Time PCR System

    • Manufacturer: Applied Biosystems
    • We have 3 units in CLIP: 3 units since 2008, 1 unit since 2013
    • They are used for a detection of minimal residual disease in acute lymphoblastic and myeloid leukemias

 

 

 

 iCycler iQ Multicolor Real-Time PCR Detection System

    • Manufacturer: Bio-Rad
    • We have 3 units in CLIP
    • We use them for a detection of minimal residual disease in acute lymphoblastic andy myeloid leukemias



 

 

 

 

Cytometric devices

 

 

CyTOF 2 Mass Cytometer

    • Manufacturer: DVS Sciences/Fluidigm
    • In CLIP since 2015
    • Allows for detection of up to 120 molecular tags per cell: http://youtu.be/TCxk_30cTL4

 

  

 

 

Cyan ADP Flow Cytometer

    • Manufacturer: Dako, Glostrup, Dennmark
    • In CLIP since 2006
    • Equipped with three lasers:
      violet (405 nm, 100 mW),
      red (642 nm, 60 mW),
      custom equipped with blue laser (488 nm, 20 mW) OBIS, Coherent, Santa Clara, CA, USA
    • Capable of measuring of 9 fluorescent parameters
    • We use this device for measuring cells and lymphocytic subpopulations, leukemias and minimal residual disease

 

 

 


 

BD FACS LSR II

    • Manufacturer: BD Bioscience, San Jose, CA, USA
    • In CLIP since 2006
    • The modified BD LSR 2 instrument is equipped with four lasers:
      standard blue (488 nm, 20 mW)
      diode red (637 nm, 140 mW) OBIS, Coherent, Santa Clara, CA, USA
      diode violet (405 nm, 120 mW) Omicron-Laserage Laserprodukte GmbH, Rodgau, Germany
      and a custom-mounted OPLS yellow-green laser (561 nm, 75 mW) Coherent, Santa Clara, CA, USA
    • This modification allows us to measure 17 fluorescence parameters
    • We use LSR II for measuring amounts of DNA, cerebrospinal fluid, PNH, EMA and for most of our research measurements

 

 


 

BD FACS Canto II

    • Manufacturer: BD Bioscience, San Jose, CA, USA
    • In CLIP since 2011
    • This device consists of three standard lasers:
      of blue (488 nm, 20 mW),
      red (637 nm, 17 mW),
      violet (405 nm, 30 mW)
    • Capable of measuring 8 fluorescence parameters
    • Tubes can be measured manually or with a carousel
    • We use FACS Canto II to measure Euroflow study, ALOT, DNA, EMA, PNH and other

 

 


 

BD FACS Aria III

    • Manufacturer: BD Bioscience, San Jose, CA, USA
    • In CLIP since 2011
    • The modified BD FACS Aria III instrument is equipped with three lasers:
      standard blue (405 nm, 50 mW),
      standard violet (405 nm, 50 mW),
      diode red (637 nm, 40 mW) OBIS, Coherent, Santa Clara, CA, USA
    • Thanks to this configuration, the device is able to measure 11 fluorescence parameters
    • FACS Aria III is used mainly for sorting of lymphocytic subpopulations

 

 


 

Cell Lab QUANTA SC MPL

    • Manufacturer: Beckman Coulter, Brea, CA, USA
    • Equipped with blue laser (488 nm, 50 mW) and with a mercury-vapor lamp (365 nm, 100 W)
    • Capable to measure 3 fluorescence parameters
    • The accurate and efficient Cell Lab Quanta SC MPL flow cytometer provides microtitre plate and micro tube based analysis
    • With Multi-Platform Loader, we can use sample cups, micro centrifuge tubes and 24-, 96- or 384-well plates.